Rat IL-6(Interleukin 6) ELISA Kit

Catalogue No BSEK-127R
Species Rat
Sensitivity 28.77 pg/mL
Range 31.25- 2000pg/mL
Size 96 T | 48 T
Price $ 365 | $ 265
Contact Us
SKU: BSEK-127R Categories: ,
Rat IL-6(Interleukin 6) ELISA Kit- Details
Uniprot ID P20607
Detection Method Sandwich
Standard 2000pg/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=100μL
Reaction time 3.5H
Research Area Tumor immunity;Endocrinology;Hormone metabolism
Alternative Names
IL-6;IL 6;IL6;Interleukin-6;Interleukin 6;Interleukin6;B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor , HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A;Hybridoma growth factor;Interferon beta-2;IFNB 2;IFNB-2;IFN-B2
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the OD of the samples to the standard curve.

Reviews

There are no reviews yet.

Be the first to review “Rat IL-6(Interleukin 6) ELISA Kit”

Your email address will not be published. Required fields are marked *