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LT-B4 (Leukotriene B4) ELISA Kit - Details |
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Alias |
LTB4 ELISA Kit, Leukotriene B4 ELISA Kit |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Competitive ELISA, Coated with Antigen |
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Detection Wavelength |
OD450 |
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Reaction Duration |
2 hours |
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Specificity |
Specifically binds with LT-B4 , no obvious cross reaction with other analogues |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Principle |
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This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with LT-B4. LT-B4 in the sample or standard competes with a fixed amount of biotin-labeled LT-B4 for sites on a pre-coated antibody specific to LT-B4. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of LT-B4 in the sample. The concentration of the target substance is inversely proportional to the OD450 value |
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