Features

• Western Or Immuno Blotting is the transfer of separated Proteins in a gel to the surface of a thin support membrane matrix.
• The Proteins are bound and immobilized on the membrane.
• Southern and Northern blotting involves the transfer of separate DNA and RNA, respectively, from a gel to a membrane
• To perform a transfer, the gel is layered next to a membrane and placed in a voltage gradient perpendicular to the gel.
• Negatively charged molecules will migrate out from the gel, move towards the positive electrode, and get deposited on the membrane.