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Violet 540 Anti-Human CD45 Antibody(HI30) - Details |
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Synonyms |
CD45|L-CA|Ly-5|Ptprc|Receptor-type tyrosine-protein phosphatase C|T200 antibody |
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Isotype |
IgG1, κ |
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Form |
Liquid |
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Conjugation |
Violet 540 |
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Conjugation Information |
Violet 540 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 548 nm (e.g., a 572/28 nm bandpass filter). |
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Clonality |
Monoclonal |
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Isotype |
IgG1, κ |
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Clone ID |
HI30 |
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UniProt ID |
P08575 |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
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Recommended dilution |
Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
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Background |
CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. |
