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Vinculin Antibody |
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Description |
Rabbit Polyclonal Antibody To Vinculin |
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Mol.Wt |
124kDa(Observed); 124kD(Calculated). |
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Uniprot |
P18206 |
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Application |
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500 |
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Clonality |
Polyclonal |
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Prediction |
Pig(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%) |
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Specificity |
Vinculin Antibody detects endogenous levels of total Vinculin. |
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Conjugate |
Unconjugated. |
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Purification |
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin. |
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Storage |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt |
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Alternative Name |
CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; OTTHUMP00000019861; OTTHUMP00000019862; VCL; VINC; VINC_HUMAN; Vinculin |
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Immunogen |
A synthesized peptide derived from human Vinculin, corresponding to a region within the internal amino acids. |
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Gene(ID) |
VCL(7414) |
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Uniprot |
P18206(VINC_HUMAN) |
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Expression |
P18206 VINC_HUMAN: Metavinculin is muscle-specific |
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Description |
Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin has been shown to be affected by the level of Actin expression |
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Subcellular Location |
Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Adherens junction. Cell junction>Focal adhesion. Cytoplasm>Cytoskeleton. Cell membrane>Sarcolemma>Peripheral membrane protein>Cytoplasmic side. |
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Tissue Specificity |
Metavinculin is muscle-specific. |
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Family & Domains |
Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion. The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. In isoform 2 (metavinculin) a 68 residue insertion in the tail domain promotes actin severing instead of bundling. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly. |
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Function |
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. |
