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Vimentin Antibody |
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Description |
Rabbit Polyclonal Antibody To Vimentin |
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Mol.Wt |
53kDa(Observed); 54kD(Calculated). |
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Uniprot |
P08670 |
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Application |
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200 |
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Prediction |
Pig(100%), Bovine(%), Horse(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%) |
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Clonality |
Polyclonal |
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Specificity |
Vimentin Antibody detects endogenous levels of total Vimentin. |
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Conjugate |
Unconjugated |
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Purification |
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin |
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Storage |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl. Store at -20 °C. Stable for 12 months from date of receipt. |
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Alternative Name |
CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; Vimentin; |
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Immunogen |
A synthesized peptide derived from human Cofilin, corresponding to a region within the internal amino acids. |
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Gene(ID) |
VIM(7431) |
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Uniprot |
P08670(VIME_HUMAN) |
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Expression |
P08670 VIME_HUMAN: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
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Description |
VIM is an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue |
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PTMs |
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
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Subcellular Location |
Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus matrix. |
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Family & Domains |
The central alpha-helical coiled-coil IF rod domain mediates elementary homodimerization. |
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Tissue Specificity |
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
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Function |
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
