Trypsin-EDTA (0.25%), phenol red

Catalog Number 25200056
Form Liquid
Source Animal Origin
Product Type Cell Culture Dissociation Reagent
Product Line Gibco™
Quantity 100 mL
Tested For In vitro Bioassay
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SKU: 25200056 Categories: ,

This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

This trypsin solution is modified as follows:

With: EDTA, Phenol Red

We offer a variety of trypsin formulations, including animal origin-free TrypLE reagents.

Specifications
Cell Type Mammalian Cells
Chelators EDTA
Concentration 1X
Osmolality 270 - 320 mOsm/kg
Shelf Life 24 Months
Shipping Condition Wet Ice
Classification Animal Origin
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Phenol Red, EDTA
pH 7.2 to 8
Unit Size Each
Storage conditions 5°C to -20°C
Manfacturer Thermo Fisher Scientific

Quality Testing

Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented Traceability

We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System

For supply chain continuity, we manufacture Gibco Trypsin-EDTA (0.25%), phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.

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