The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Specifications
| Method | Filtered through 1-micron filter. |
| Storage | Room Temp. |
| Synonym | TAE |
| pH | 8.40 ± 0.20 (1X) |
| QC Testing | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
| Tris-Base | 77-86-1 | 121.14 | 2 M |
| Acetic Acid, Glacial | 64-19-7 | 60.05 | 1 M |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 50 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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