| Testosterone ELISA Kit | |
| Sensitivity | 0.09 ng/mL |
| Range | 0.16-10ng/mL |
| Alternative Names | T; Testesteron; Testeronun |
| Detection Method | Competitive |
| Standard | 10ng/mL |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL |
| Reaction time | 1.5H |
| Research Area | Signaling transduction；Cancer；Immunology；Metabolism |
| Principle | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to T. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Testosterone ELISA Kit | |
| Sensitivity | less than 1.95pg/mL |
| Range | 3.9pg/mL-250pg/mL |
| Detection Method | Competitive |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL |
| Alternative Names | Testosterone |
| Specificity | Recombinant and Natural General Testosterone |
| Principle | The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General Testosterone, During the reaction, General Testosterone in the sample or standard competes with a fixed amount of biotin-labeled General Testosterone for sites on a pre-coated Monoclonal antibody specific to General Testosterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General Testosterone in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Testosterone Elisa Kit
| Catalogue No | BSEK-336U |
| Species | Universal |
| Size | 96 T | 48 T |
| Price | POR |
This immunoassay kit allows for the in vitro quantitative determination of General Testosterone concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.