SYBR Green PCR Super Mix is an optimized 2x real-time PCR master mix containing Hot Star Taq DNA Polymerase, SYBR Green® fluorescent dye, dNTP and Mg²⁺. In addition, the balanced K⁺ and NH4⁺ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. The unique PCR buffer ensures sensitive qPCR on all real-time PCR instruments without optimization.

A reaction system as described below was established. To perform multiple reactions, prepare a pre mix of the common components, add a suitable volume to each tube or well, and then adda special reaction component