Note: This reagent is a strong lysate, which can extract nuclear protein, but at the same time of extracting nuclear protein, the genome will also be released, resulting in a thick cell lysate. At this time, the protein sample buffer can be directly added, boiled and centrifuged, and the sample is directly loaded after centrifugation. To determine the concentration, add a small amount of SDS (1%), boil and centrifuge the concentration. The protein extracted by this series of protein extraction reagents contains stain remover, so it is not suitable for Bradford protein concentration determination kit. Please choose BCA method or Lowry method to detect protein concentration. If a protein that is particularly tightly bound to the genome needs to be detected, the goo can be broken up by ultrasound treatment, then the supernatant can be centrifuged for subsequent experiments.