| Ribonuclease A - Specifications | |
| Synonyms | Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase |
| Grade | Molecular Biology Grade |
| Application Notes | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. |
| Biochemical Actions | Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides. |
| Extinction Coefficient | E1%280 = ~7.3 |
| Format | Lyophilized powder, Salt-free and protease-free |
| Hazard Statements | H317 |
| Isoelectric Point | 9.45 |
| Protective Equipment | Eyeshields, Gloves, respirator filter |
| pH | Optimum pH: 7.0 - 7.5 |
| Preparation Method | Chromatographically Purified |
| Solubility | Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution. |
| Source | Bovine Pancrease |
| Specific Activity | ≥70 Kunitz units/mg |
| Specificity | The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains. |
| Unit Definition | One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Size | 10 MG | 50 MG | 250 MG | 500 MG | 1 GM |
| HSN Code | 293499.00 |
Ribonuclease A
CAS NO: 9001-99-4
| Catalogue No | BSCHM-012R |
| Mol. Formula | N/A |
| Mol. Weight | N/A |
| Price | POR |
Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples.