| Alternative Names |
total SOD ELISA Kit, superoxide dismutase ELISA Kit |
| Detection Method |
Sandwich ELISA, Double Antibody |
| Storage |
2-8°C(Sealed), Don’t cryopreserve. |
| Specificity |
Specifically binds with NOX4 , no obvious cross reaction with other analogues. |
| This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti total SOD antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with total SOD bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of total SOD in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
| Precautions |
The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information. |
| Documents |
Product manual with the material |
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