Purpose

Our Rat Substance P (SP) ELISA Kit is to assay SP levels in Rat serum, plasma, culture media or any biological fluid

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to SP. Standards or samples are
added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for SP is added to each
Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain SP and HRP conjugated SP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SP. You can calculate the concentration of SP in the samples by comparing the OD of the samples to the standard curve.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rat SP were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rat SP were tested on 3 different plates, 8 replicates in each plate

CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%