Our Rat Nitric Oxide (NO) ELISA kit is to assay NO levels in Rat serum, plasma, culture media or any biological fluid.
| Rat Nitric Oxide ELISA Kit - Details | |
| Standard | 45μmol/L |
| Wavelength | 450nm |
| Detection Method | Sandwich-ELISA |
| Sample Volume | 10ul |
| Sample type | serum, plasma, culture media, or any biological fluids. |
| Storage | 2-8℃ |
| Test Principle | |
| This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to NO. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for NO is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NO and HRP conjugated NO antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NO. You can calculate the concentration of NO in the samples by comparing the OD of the samples to the standard curve. | |
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