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Rat Mycoplasma Pulmonis IgG (MP-IgG) ELISA Kit |
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Sensitivity |
- |
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Range |
- |
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Method |
Indirect ELISA |
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Detection Method |
Qualitative Detection |
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Qualitative |
Negative/Positive |
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Sample Type |
For use with serum, plasma, culture media, or any biological fluids. |
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Wavelength |
450nm |
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Sample Volume |
10ul |
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Storage |
2-8℃ |
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Principle |
The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an antigen specific to MP-IgG, make it to solid-phase antigen. Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for MP-IgG is added to each Microplate well and incubated, so the antigen-antibody-Enzyme labeled antigen complex is formed .Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain MP-IgG and HRP conjugated MP antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of MP-IgG is determined by comparing with the CUT OFF value |
