|
Rat MT(Melatonin) ELISA Kit |
|
Sensitivity |
2.78 pg/mL |
|
Range |
7.82-500 pg/mL |
|
Detection Method |
Competitive Inhibition |
|
Sample Type |
cell lysates and other biological fluids |
|
Alternative Names |
N-Acetyl-5-Methoxytryptamine |
|
Assay length |
2.5h |
|
Standard |
500 pg/mL |
|
Research Area |
Endocrinology |
|
Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve. |
