Enzyme immunoassay for the quantitative detection of Rat IL-8 antigen in serum, plasma, culture media, or any biological fluid. Our Rat Interleukin 8 (IL-8)ELISA Kit is to assay IL-8 levels in Rat serum, plasma, culture media or any biological fluid.
| Rat Interleukin 8 (IL-8)ELISA Kit - Details | |
| Standard | 2250pg/ml |
| Wavelength | 450nm |
| Detection Method | Sandwich-ELISA |
| Sample Volume | 10ul |
| Sample type | serum, plasma, culture media, or any biological fluids. |
| Storage | 2-8℃ |
| Test Principle | |
| This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to IL-8 Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for IL-8 is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-8 and HRP conjugated IL-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-8 You can calculate the concentration of IL-8 in the samples by comparing the OD of the samples to the standard curve. | |
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