| Catalogue No. | HS-EL0033Ra |
| Size | 48T | 96T |
| Detection Range | 7.81pg/mL-500pg/mL |
| Sensitivity | 1.8 pg/mL |
| Test Method | Sandwich Method |
| Sample Type | Serum | plasma | cell culture supernatant and other biological samples |
| PLATFORM | ELISA |
|---|---|
| PLATE | Detachable 96-well plate |
| SIZE | 96T/48T |
| STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| DELIVERY | 4℃ blue ice transportation |
This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique. Specific anti-rat IL-6 antibodies are precoated on a high-affinity ELISA plate. Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-6 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
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