|
Rat IL-1β(Interleukin 1 Beta) ELISA Kit - Details |
|
Product Name |
Rat IL-1β(Interleukin 1 Beta) ELISA Kit |
|
Uniprot ID |
Q63264 |
|
Detection Method |
Sandwich |
|
Standard |
1000pg/mL |
|
Sample Type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
|
Reaction Time |
3.5H |
|
Research Area |
Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science |
|
Alternative Names |
Catabolin; IL1β;IL-1β;IL1-β;IL 1β;IL1 β;IL 1 β;I L1β;IL 1 BETA;IL1BETA;IL-1BETA;IL1-BETA;IL 1BETA;IL1 BETA;IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta; IL1β; IL1b;nterleukin-1 Family Member 2 |
|
Test Principle |
|
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the OD of the samples to the standard curve. |
Reviews
There are no reviews yet.