Description
Standard Curve
Storage2-8 ℃ for 6 monthsRecovery
Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples.
| Matrix | Recovery range(%) | Average(%) |
|---|---|---|
| serum(n=5) | 85-102 | 95 |
| EDTA plasma(n=5) | 86-104 | 94 |
| heparin plasma(n=5) | 87-103 | 97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=5) | 85-105% | 89-104% | 86-105% |
| EDTA plasma(n=5) | 88-99% | 85-100% | 82-98% |
| heparin plasma(n=5) | 89-99% | 80-100% | 81-100% |
CV(%)Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note : For Research Use Only
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