Detection Method: Sandwich ELISA, Double Antibody
Application: IL-18 ELISA Kit allows for the in vitro quantitative determination of IL-18 concentrations in serum, plasma, tissue homogenates and other biological fluids
Alias: IL-18 ELISA Kit, Interleukin 18 ELISA Kit, IL18 ELISA Kit, IGIF ELISA Kit, IL-1g ELISA Kit, IL-1F4 ELISA Kit, Interferon-gamma-inducing factor ELISA Kit
Storage: 2-8 ℃ for 6 months
Recovery: Matrices listed below were spiked with certain level of IL-18 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-18 in samples.
| Matrix | Recovery range(%) | Average(%) |
|---|---|---|
| serum(n=5) | 85-101 | 96 |
| EDTA plasma(n=5) | 88-103 | 94 |
| heparin plasma(n=5) | 89-105 | 99 |
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=5) | 89-98% | 87-105% | 87-103% |
| EDTA plasma(n=5) | 82-95% | 82-88% | 87-96% |
| heparin plasma(n=5) | 81-88% | 80-97% | 83-99% |
CV(%)Intra-Assay: CV<8%
Inter-Assay: CV<10%
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