|
Rat Hyp(Hydroxyproline) ELISA Kit |
|
Sensitivity |
27.3 ng/mL |
|
Range |
78.13-5000 ng/mL |
|
Reactivity |
Rat |
|
Detection Method |
Competitive Inhibition |
|
Sample Type |
serum, plasma, cell lysates, cell culture supernates and other biological fluids |
|
Alternative Names |
(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N) |
|
Assay length |
2h |
|
Standard |
5000 ng/mL |
|
Research Area |
Metabolic pathway;Nutrition metabolism |
|
Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Hyp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Hyp in the samples is then determined by comparing the OD of the samples to the standard curve. |
|
Validated Image |
|
