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Rat 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit |
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Sensitivity |
0.94ng/mL |
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Range |
1.56ng/mL -100 ng/mL |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Storage |
2-8℃. |
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Intended Use |
For the quantitative determination of Rat 8-Hydroxydeoxyguanosine(8-OHdG) concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates and other biological fluids |
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Principle |
The kit was used to test the level of Rat 8-Hydroxydeoxyguanosine(8-OHdG), based on the principle of double antibody sandwich technology enzyme linked immunosorbent assay (ELISA).
Add Standard and Sample to the wells that pre-coated with objective antibody, then add HRP-Conjugate reagent to form an immune complex, incubation, by incubation and washing, removal of unbound enzyme, and then add the substrate A and B, then the solution will turn blue and finally change into yellow at the effect of acid. The color depth or light was positively correlated with the concentration of 8-Hydroxydeoxyguanosine (8-OHdG) |