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Rat 5-HT(5-Hydroxytryptamine) ELISA Kit |
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Sensitivity |
3.81 ng/mL |
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Range |
14.07-900 ng/mL |
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Detection Method |
Competitive Inhibition |
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Sample Type |
serum, plasma |
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Alternative Names |
5HT; Serotonin; N-Methyl-Gamma |
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Assay length |
2h |
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Standard |
900 ng/mL |
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Research Area |
Signal transduction;Infection immunity;Neuro science;Gastroenterology |
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Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve. |
