| Rat Hspa5 (78 kDa glucose-regulated protein) ELISA Kit | |
| Sensitivity | 0.17 ng/mL |
| Range | 0.32-20 ng/mL |
| Detection Method | Sandwich |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative Names | 78 kDa glucose-regulated protein; GRP-78; Binding-immunoglobulin protein; BiP; HSP70 family protein 5; Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; Endoplasmic reticulum chaperone BiP; BIP |
| Assay length | 3.5h |
| Standard | 20 ng/mL |
| Uniprot ID | P06761 |
| Research Area | Signal transduction;Cardiovascular biology;Reproductive science; Neuro science |
| Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Hspa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hspa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Hspa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Hspa5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
