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Rat High Sensitivity C Reactive Protein (hs-CRP) Elisa Kit |
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Sensitivity |
0.16 µg/mL |
|
Range |
0.78-50 µg/mL |
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Detection Method |
Sandwich |
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Sample Type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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Alternative Names |
C-RP; PTX1; Pentraxin-Related; Pentraxin 1 |
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Assay length |
3.5h |
|
Standard |
50 µg/mL |
|
Research Area |
Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology |
|
Uniprot ID |
P48199 |
|
Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve. |
