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Rat GSH(Glutathione) ELISA Kit - Details |
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Alternative Names |
L-Reduced Glutathione |
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Detection Method |
Competitive Inhibition |
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Standard |
100 µg/mL |
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Sample Type |
serum, plasma, cell lysates, cell culture supernates and other biological fluids |
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Reaction time |
2h |
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Research Area |
Metabolic pathway |
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Test Principle |
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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GSH in the samples is then determined by comparing the OD of the samples to the standard curve. |
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