Description
| Product Name |
Rat Fndc5(Fibronectin type III domain-containing protein 5) ELISA Kit |
| Detection Method |
Sandwich ELISA, Double Antibody |
| Application |
Fndc5 ELISA Kit allows for the in vitro quantitative determination of Fndc5 concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Species |
Rat |
| Standard Curve |
 |
| Storage |
2-8 ℃ for 6 months |
| Recovery |
Matrices listed below were spiked with certain level of Fndc5 and the recovery rates were calculated by comparing the measured value to the expected amount of Fndc5 in samples.
| Matrix |
Recovery range(%) |
Average(%) |
| serum(n=5) |
88-104 |
98 |
| EDTA plasma(n=5) |
86-105 |
95 |
| heparin plasma(n=5) |
88-98 |
95 |
|
| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fndc5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample |
1:2 |
1:4 |
1:8 |
| serum(n=5) |
88-102% |
85-100% |
86-98% |
| EDTA plasma(n=5) |
84-97% |
87-98% |
84-98% |
| heparin plasma(n=5) |
85-100% |
81-97% |
83-99% |
|
| CV (%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
| Note |
For Research Use Only |
| For any quries, please write to us at info@bio-string.com |
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