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Rat 2,3-Diphosphoglycerate (2,3-DPG) Elisa Kit |
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Sensitivity |
0.05umol/ml |
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Range |
0.10 umol/ml -0.156 umol/ml |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Storage |
2-8℃. |
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Intended Use |
For the quantitative determination of Rat 2,3-Diphosphoglycerate(2,3-DPG) concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates and other biological fluids. |
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Principle |
The kit was used to test the level of Rat 2,3-Diphosphoglycerate(2,3-DPG), based on the principle of double antibody sandwich technology enzyme linked immunosorbent assay (ELISA).
Add Standard and Sample to the wells that pre-coated with objective antibody, then add HRP-Conjugate reagent to form an immune complex, incubation, by incubation and washing, removal of unbound enzyme, and then add the substrate A and B, then the solution will turn blue and finally change into yellow at the effect of acid. The color depth or light was positively correlated with the concentration of 2,3-Diphosphoglycerate (2,3-DPG). |
