Description
Product Name |
Rat Ctsb(Cathepsin B) ELISA Kit |
Catalogue No. |
4920RE |
Detection Method |
Sandwich ELISA, Double Antibody |
Application |
Ctsb ELISA Kit allows for the in vitro quantitative determination of Ctsb concentrations in serum, plasma, tissue homogenates and other biological fluids. |
Species |
Rat |
Standard Curve |
|
Storage |
2-8 ℃ for 6 months |
Recovery |
Matrices listed below were spiked with certain level of Ctsb and the recovery rates were calculated by comparing the measured value to the expected amount of Ctsb in samples.
Matrix |
Recovery range(%) |
Average(%) |
serum(n=5) |
93-103 |
98 |
EDTA plasma(n=5) |
86-100 |
93 |
heparin plasma(n=5) |
87-100 |
94 |
|
Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ctsb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
serum(n=5) |
86-105% |
87-102% |
87-99% |
EDTA plasma(n=5) |
84-100% |
84-90% |
82-100% |
heparin plasma(n=5) |
89-97% |
85-97% |
81-98% |
|
CV (%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
Note |
For Research Use Only |
Contact us for help
info@bio-string.com |
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