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Rat CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit |
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Sensitivity |
0.7 ng/mL |
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Range |
1.57-100 ng/mL |
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Detection Method |
Sandwich |
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Sample Type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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Alternative Names |
CK2; CK-MB; Creatine Kinase,Muscle/Brain |
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Assay length |
3.5h |
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Standard |
100 ng/mL |
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Research Area |
Enzyme & Kinase;Cardiovascular biology; |
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Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CKMB in the samples is then determined by comparing the OD of the samples to the standard curve. |
