Description
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Calprotectin Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Calprotectin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Calprotectin and HRP conjugated Calprotectin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Calprotectin You can calculate the concentration of Calprotectin in the samples by comparing the OD of the samples to the standard curve.
| Catalogue No. | EKR-1072Ra |
| Size | 96T |
| Product Category | ELISA (Quantitative) |
| Reactivity | Rat |
| Sample | Serum, urine, tissue, plasma, cell culture supernatants, body fluid and tissue homogenate |
| Assay Type: | Sandwich-ELISA |
| Assay Length | 3.5h |
| Sensitivity | 0.1 ng/ml |
| Range | 0.65-400ng/ml |
| Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Precautions | The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information.info@bio-string.com |
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