| Product Name |
Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit |
| Detection Method |
Sandwich ELISA, Double Antibody |
| Application |
BDNF ELISA Kit allows for the in vitro quantitative determination of BDNF concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Species |
Rat |
| Standard Curve |
 |
| Storage |
2-8 ℃ for 6 months |
| Recovery |
Matrices listed below were spiked with certain level of BDNF and the recovery rates were calculated by comparing the measured value to the expected amount of BDNF in samples.
| Matrix |
Recovery range(%) |
Average(%) |
| serum(n=5) |
91-104 |
98 |
| EDTA plasma(n=5) |
86-104 |
93 |
| heparin plasma(n=5) |
89-105 |
100 |
|
| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample |
1:2 |
1:4 |
1:8 |
| serum(n=5) |
85-105% |
85-104% |
87-105% |
| EDTA plasma(n=5) |
83-94% |
83-97% |
84-101% |
| heparin plasma(n=5) |
84-100% |
80-96% |
80-100% |
|
| CV (%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
| Note |
For Research Use Only |
| For any quries, please write to us at info@bio-string.co |
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