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Rat anti-PEG(Polyethylene glycol) IgM ELISA Kit |
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Sensitivity |
0.094ng/ml |
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Range |
0.156-10ng/ml |
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Alternative Name |
PEG IgM ELISA Kit, PEG(Polyethylene glycol) IgM ELISA Kit |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Indirect ELISA |
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Wavelength |
OD450 |
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Reaction Duration |
3 hours |
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Specificity |
Specifically binds with Anti-PEG IgM , no obvious cross reaction with other analogues. |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Principle |
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with Anti-PEG IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-PEG IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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