Application: Ace2 ELISA Kit allows for the in vitro quantitative determination of Ace2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Standard Curve
Recovery: Matrices listed below were spiked with certain level of Ace2 and the recovery rates were calculated by comparing the measured value to the expected amount of Ace2 in samples.
Matrix | Recovery range(%) | Average(%) |
---|---|---|
serum(n=5) | 88-103 | 97 |
EDTA plasma(n=5) | 88-104 | 97 |
heparin plasma(n=5) | 92-103 | 98 |
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ace2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
---|---|---|---|
serum(n=5) | 86-104% | 90-101% | 85-104% |
EDTA plasma(n=5) | 87-97% | 83-100% | 82-101% |
heparin plasma(n=5) | 83-99% | 81-97% | 81-98% |
CV(%)Intra-Assay: CV<8%
Inter-Assay: CV<10%
No
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