| Rat 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit | |
| Sensitivity | 0.46 ng/mL |
| Range | 1.56-100 ng/mL |
| Alternative Names | 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine |
| Assay Type | Competitive Inhibition |
| Standard | 100 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2h |
| Research Area | Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology; |
| Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve. |