|
Product Name |
Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit |
|
Standard |
100 ng/mL |
|
Sample Type |
serum, plasma and other biological fluids |
|
Assay Length |
2h |
|
Research Area |
Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology; |
Standard Curve
|
Concentration (ng/mL) |
OD |
Corrected OD |
|
100.00 |
0.189 |
- |
|
50.00 |
0.332 |
- |
|
25.00 |
0.621 |
- |
|
12.50 |
0.897 |
- |
|
6.25 |
1.253 |
- |
|
3.13 |
1.583 |
- |
|
1.57 |
1.761 |
- |
|
0.00 |
2.211 |
- |
Precision
- Intra-assay Precision (Precision within an assay)：CV%<8%
- Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
- Inter-assay Precision (Precision between assays)：CV%<10%
- Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
|
Matrix |
Range(%) |
Average Recovery(%) |
|
Serum (n=8) |
78-92% |
85% |
|
EDTA plasma (n=8) |
87-99% |
93% |
|
Heparin plasma(n=5) |
80-95% |
97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Matrix |
1:2 |
1:4 |
1:8 |
1:16 |
|
Serum (n=5) |
81-94% |
96-105% |
88-106% |
93-102% |
|
EDTA plasma(n=5) |
95-104% |
97-106% |
85-98% |
81-92% |
|
Heparin plasma(n=5) |
95-106% |
87-105% |
92-101% |
79-90% |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
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