| Product Name |
Rat 5-HT(5-Hydroxytryptamine) ELISA Kit |
| Catalogue No. |
4598ELK |
| Alternative Names: |
5HT; Serotonin; N-Methyl-Gamma |
| Assay Type |
Competitive Inhibition |
| Sensitivity |
3.97 ng/mL |
| Range |
14.07-900 ng/mL |
| Sample Type |
serum, plasma, tissue homogenates |
| Assay Length |
2h |
| Research Area |
Signal transduction;Infection immunity;Neuro science;Gastroenterology; |
| Test principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine(5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine(5-HT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine(5-HT) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Standard Curve |
| Concentration (ng/mL) |
OD |
Corrected OD |
| 900.00 |
0.232 |
— |
| 450.00 |
0.397 |
— |
| 225.00 |
0.557 |
— |
| 112.50 |
0.993 |
— |
| 56.25 |
1.197 |
— |
| 28.13 |
1.596 |
— |
| 14.07 |
1.871 |
— |
| 0.00 |
2.101 |
— |
|
| Precision |
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
| Recovery |
Matrices listed below were spiked with certain level of recombinant 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HT in samples.
| Matrix |
Recovery range |
Average |
| serum(n=5) |
80-93% |
86% |
| EDTA plasma(n=5) |
94-105% |
98% |
| Heparin plasma(n=5) |
95-107% |
89% |
|
| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix |
1:2 |
1:4 |
1:8 |
1:16 |
| serum(n=5) |
79-94% |
87-96% |
98-105% |
82-93% |
| EDTA plasma(n=5) |
95-102% |
93-101% |
97-105% |
95-103% |
| Heparin plasma(n=5) |
78-96% |
86-92% |
84-98% |
89-102% |
|
| Note |
For Research Use Only |
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