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Raptor Antibody |
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Description |
Rabbit polyclonal antibody to Raptor |
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Cited expt |
WB, IHC |
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Mol.Wt |
149kDa(Observed); 149kD(Calculated). |
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Uniprot |
Q8N122 |
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Application |
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500 |
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Prediction |
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Dog(%), Chicken(%) |
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Clonality |
Polyclonal |
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Specificity |
Raptor Antibody detects endogenous levels of total Raptor. |
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Conjugate |
Unconjugated. |
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Purification |
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin. |
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Storage |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. |
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Alternative Name |
KIAA1303; KOG1; Mip1; P150 target of rapamycin (TOR) scaffold protein; p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Raptor; Regulatory associated protein of mTOR; Regulatory associated protein of MTOR complex 1; Regulatory-associated protein of mTOR; RPTOR; RPTOR_HUMAN |
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Gene(ID) |
RPTOR(57521) |
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Expression |
Q8N122 RPTOR_HUMAN Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen. |
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PTMs |
Insulin-stimulated phosphorylation at Ser-863 by MTOR and MAPK8 up-regulates mTORC1 activity. Osmotic stress also induces phosphorylation at Ser-696, Thr-706 and Ser-863 by MAPK8. Ser-863 phosphorylation is required for phosphorylation at Ser-855 and Ser-859. In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1, which stimulates mTORC1 activity. |
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Subcellular Location |
Cytoplasm. Lysosome. Cytoplasmic granule |
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Tissue Specificity |
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen. |
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Family & Domains |
Belongs to the WD repeat RAPTOR family |
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Function |
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. |
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Validated Image |
Western blot analysis of lysates from 293 cells(LPS 4h treatment), using Raptor Ab. The lane on the left was treated with blocking peptide.
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