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Random Hexamers

Random Hexamers

Catalouge No BSRC-36-1
Category cDNA Synthesis
Size 50µM
Price POR
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Reverse transcription is a crucial step in many molecular biology applications, including gene expression analysis, RNA sequencing, and viral detection. A key component in this process is the primer used to initiate cDNA synthesis.  Random Hexamers are a high-quality mixture of six-nucleotide sequences designed to bind randomly across RNA templates, enabling unbiased and efficient first-strand cDNA synthesis.

Supplied at a ready-to-use 50 µM concentration, Random Hexamers provide flexibility for various reverse transcription reactions. Their high purity and optimized formulation enhance the efficiency of reverse transcriptase enzymes, ensuring comprehensive RNA coverage and minimizing bias. These primers are particularly useful when working with complex RNA populations, degraded RNA samples, or full-length transcript analysis.

Random Hexamers are compatible with various reverse transcription systems and are ideal for applications such as RT-qPCR, cDNA library preparation, and NGS workflows. By facilitating uniform priming, they improve the accuracy and reproducibility of downstream assays.

With Enzymes’ commitment to quality and performance, our Random Hexamers are manufactured under stringent quality control conditions to ensure batch-to-batch consistency. Whether you are working on gene expression studies, pathogen detection, or transcriptome analysis, Random Hexamers offer a reliable solution for efficient and unbiased reverse transcription.

Single Pack Contents

Random Hexamers (50µM): 50µl X 5 vials

Random hexamers can be used for:

  • cDNA synthesis by priming reverse transcription of RNA into complementary DNA (cDNA).
  • Labeling and amplification of nucleic acids in hybridization-based assays and PCR.
  • Generating diverse cDNA libraries from various RNA templates for transcriptome analysis.

 

Incubation of random hexamers at 25℃ for 10-15 minutes improves cDNA yields.