Specificity
This assay has high sensitivity and excellent specificity for detection of Quinolinic Acid (QA). No significant cross-reactivity or interference between Quinolinic Acid (QA) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Quinolinic Acid (QA) and the recovery rates were calculated by comparing the measured value to the expected amount of Quinolinic Acid (QA) in samples.
| Matrix | Recovery Range(%) | Average Recovery(%) |
| serum(n=5) | 90-98 | 94 |
| EDTA plasma(n=5) | 81-99 | 93 |
| heparin plasma(n=5) | 90-98 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Quinolinic Acid (QA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-101% | 80-99% | 96-105% | 89-96% |
| EDTA plasma(n=5) | 93-101% | 84-96% | 99-105% | 83-104% |
| heparin plasma(n=5) | 81-99% | 91-102% | 94-105% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to QA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled QA and unlabeled QA (Standards or samples) with the pre-coated antibody specific to QA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of QA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of QA in the sample.
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