Protein G-Peroxidase from Streptococcus sp

Catalouge No P8170
Synonyms Protein G−HRP from Streptococcus sp.
Quality Level 200
Form Lyophilized Powder
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SKU: P8170 Categories: ,
Recombinant Expressed in Unspecified Host
Conjugate Peroxidase Conjugate
Technique(s) direct ELISA: 1:100,000 using human IgG
Storage Temp. −20°C
Manufacturer Sigma-Aldrich Solutions

General Description

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes

Application

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.

Biochem/physiol Actions

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

Preparation Note

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett.95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

MDL Number: MFCD00165575
UNSPSC Code: 12352203
NACRES: NA.46

Storage Class Code: 11 - Combustible Solids

WGK: WGK3

Flash Point(F): Not applicable

Flash Point(C): Not applicable

Personal Protective Equipment: dust mask type N95 (US), Eye shields, Gloves

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