|
Progesterone (PG) ELISA Kit |
|
Sensitivity |
46.88 pg/mL |
|
Range |
78.13-5000pg/mL |
|
Uniprot ID |
N/A |
|
Alternative Names |
Progesterone, Pg, P4 |
|
Detection Method |
Competitive |
|
Standard |
5000 pg/mL |
|
Sample Type |
Serum, Plasma, Other biological fluids,Sample volume: 50μL |
|
Reaction Time |
1.5H |
|
Research Area |
Cardiovascular；Signaling transduction；Neuroscience；Cancer；Metabolism |
|
Principle |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Progesterone(PG). Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Progesterone(PG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Progesterone(PG) in the samples is then determined by comparing the OD of the samples to the standard curve. |
|
Validated Image |
|
|
Progesterone (PG) ELISA Kit |
|
Sensitivity |
0.49 ng/mL |
|
Range |
1.57-100 ng/mL |
|
Alternative Names |
P4; Pregn-4-Ene-3,20-Dione |
|
Detection Method |
Competitive Inhibition |
|
Standard |
100 ng/mL |
|
Sample Type |
serum, plasma and other biological fluids |
|
Reaction Time |
2h |
|
Research Area |
Endocrinology;Reproductive science;Hormone metabolism |
|
Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PG in the samples is then |
|
Validated Image |
|
|
Progesterone (PG) ELISA Kit |
|
Sensitivity |
0.056ng/mL |
|
Range |
0.156-10ng/mL |
|
Detection Method |
Competitive |
|
Alternative Names |
Progesterone, Pg, P4 |
|
Sample Type |
Serum, Plasma, Other biological fluids,Sample volume: 50μL |
|
Principle |
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General Progesterone, During the reaction, General Progesterone in the sample or standard competes with a fixed amount of biotin-labeled General Progesterone for sites on a pre-coated Monoclonal antibody specific to General Progesterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General Progesterone in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
|
Intended Use |
This immunoassay kit allows for the in vitro quantitative determination of General Progesterone concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. |
