Phusion High-Fidelity DNA Polymerases (2 U/μL)

Catalog Number F530S
Product Type High-Fidelity DNA Polymerase
Color Colorless
No. of Reactions 100 Reactions
Reaction Format Standalone
Reaction Speed Fast
Polymerase Phusion High-Fidelity DNA Polymerase
Quantity 100 units
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Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)
Specifications
Overhang Blunt
Fidelity (vs. Taq) 52X
Hot Start No
Shipping Condition Dry Ice
Size (Final Product) 20 kb or less
Concentration 2 U/μL
Sterility Sterile-filtered
For Use With (Application) Standard PCR, High-fidelity PCR
GC-Rich PCR Performance High
Unit Size Each
Manfacturer Thermo Fisher Scientific

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

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