Phospho-RPA32/RPA2 (Thr21) Recombinant Rabbit mAb

Catalogue No BAAB-011P
Reactivity Human, Mouse, Rat
Host Rabbit
Tested Application WB,IP
Amount 100ul
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SKU: BAAB-011P Categories: ,
Altername REPA2; RPA32; RP-A p32; RP-A p34
Recommended Dilutions WB: 1:10000 | IP: 1:20
Conjugate Unconjugated
Purification Affinity Purification
Specificity IgG
Molecular Weight 32 kDa
Storage/Stability Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Formulation Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Immunogen A synthetic phosphopeptide corresponding to residues surrounding Thr21 of human RPA32/RPA2
Modification Phosphorylated

Background

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and coordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in coordinating different damage response pathways.

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