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PerCP-Cy5.5 Anti-Mouse CD62L Antibody (MEL-14) |
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Alternative Names |
CD62L|CD62 antigen-like family member L|L-selectin|LAM-1|LECAM1|Lnhr|Ly-22|Ly22|Lymph node homing receptor|Sell antibody |
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Isotype |
IgG2a, κ |
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Form |
Liquid |
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Conjugation |
PerCP-Cy5.5 |
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Clonality |
Monoclonal |
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Clone ID |
MEL-14 |
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UniProt ID |
P18337 |
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Conjugation Information |
PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
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Recommended dilution |
Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
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Background |
CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte 'rolling' on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1. |
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Validated Image |
Staining of C57BL/6 murine splenocytes with PerCP-Cy5.5 Anti-Mouse CD62L Antibody(Mel14) (filled gray histogram) or PerCP-Cy5.5 Rat IgG2a, κ Isotype Control (black histogram). Total viable cells were used for analysis
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