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PerCP-Cy5.5 Anti-Human/Mouse CD44 Antibody (IM7) |
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Alternative Names |
MDU2MDU3MIC4|CD44|CD44 antigen|CDw44|Epican|LHR|PGP-1|PGP-I|Phagocytic glycoprotein 1|Phagocytic glycoprotein I antibody |
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Isotype |
IgG2b, κ |
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Form |
Liquid |
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Conjugation |
PerCP-Cy5.5 |
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Clonality |
Monoclonal |
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Clone ID |
IM7 |
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UniProt ID |
P15379 P16070 |
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Conjugation Information |
PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
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Recommended dilution |
Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
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Background |
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. |
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Validated Image |
C57BL/6 murine splenocytes are stained with PerCP-Cy5.5 Anti-Human/Mouse CD44 Antibody (filled red histogram). Unstained splenocytes (empty black histogram) are used as control.
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