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PE-Cy5 Anti-Mouse CD3 Antibody(17A2) - Details |
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Synonyms |
CD3|CD3E/D/G/Z|CD3e/d/g/z|T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain antibody |
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Clonality |
Monoclonal |
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Isotype |
IgG2b, κ |
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Form |
Liquid |
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Conjugation |
PE-Cy5 |
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Conjugation Information |
PE-Cy5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter). |
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Clone ID |
17A2 |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
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Alternative Names |
CD3|CD3E/D/G/Z|CD3e/d/g/z|T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain antibody |
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UniProt ID |
P04235 P11942 P22646 P24161 |
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Recommended dilution |
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Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
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Background |
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CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
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