OmniPCR (OnePCR Ultra)Ā is a ready-to-use combined solution containing the Thermostable DNA polymerase, PCR buļ¬er, dNTPs, gel loading dyes, enhancer, andĀ fluorescence dye. Directly add primers, template, and water to execute the polymerase chain reaction (PCR). The Taq DNA polymerase exhibits 5ā²-exonuclease activity and after carrying DNA electrophoresis, the OmniPCR (OnePCR Ultra)Ā fluorescence dye is directly detected onĀ BluPAD LED transilluminatorĀ or other compatible transilluminator. The supermix is provided at 2X concentration and used at 1X concentration for the addition of primer and template solutions. Reagents are provided with the suļ¬cient amplification reactions of 50 Ī¼l each, 100 reactions per kit.
Instrument Compatibility
This Super Mix is compatible with the majority of commercially available PCR systems.
Reaction Setup
As a starting point, please place the pre-chilled components on ice and follow the below steps:
Standard PCR with OmniPCR (OnePCR Ultra):
| 1. For each 50 Ī¼l reaction, assemble the following components in a 0.2 ml PCR tube on ice just prior to use: | |
| OmniPCR (OnePCR Ultra) | 25 |
| Forward primer, 5ā¼10 uM | Variable |
| Reverse primer, 5ā¼10 uM | Variable |
| DNA template | Variable |
| Add ddH2O to | 50 |
| 2. Mix gently. If necessary, centrifuge brieļ¬y. Cap tubes and place in the thermal cycler. | |
| 3. Process in the thermal cycler for 25ā¼35 cycles as follows: | |
| Initial Denaturation | 2ā¼5 minutes at 94Ā°C |
| Denaturation | 20ā¼40 seconds at 94Ā°C |
| Annealing | 1 min at the proper annealing temperature |
| Extension | 2 min at 72Ā°C |
| Final extension | 5 min at 72Ā°C |
After the PCR reaction, perform agarose electrophoresis to detect PCR product. No additional dye is required for the PCR samples. GelsĀ canĀ be post-stained with Ethidium Bromide if desired.
Use the blue-light or UV transilluminator to photograph the gel
Important Notes:Ā OmniPCR Supermix w Fluorescent dye is light sensitive and should be stored and protected from light
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